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Open AccessReview
Sustainable, Renewable and Environmental-Friendly Insulation Systems for High Voltages Applications
Molecules 2020, 25(17), 3901; https://doi.org/10.3390/molecules25173901 (registering DOI) - 27 Aug 2020
Abstract
With the inception of high voltage (HV), requisites on the insulating permanence of HV equipment is becoming increasingly crucial. Mineral/synthetic oil liquid insulation—together with solid insulation materials (paper, pressboard)—is the fundamental insulation constituent in HV apparatuses; their insulation attributes perform a substantial part [...] Read more.
(This article belongs to the Special Issue Dielectric Materials: Challenges and Prospects)
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Open AccessArticle
Efficient and Versatile Modeling of Mono- and Multi-Layer MoS2 Field Effect Transistor
Electronics 2020, 9(9), 1385; https://doi.org/10.3390/electronics9091385 (registering DOI) - 27 Aug 2020
Abstract
Two-dimensional (2D) materials with intrinsic atomic-level thicknesses are strong candidates for the development of deeply scaled field-effect transistors (FETs) and novel device architectures. In particular, transition-metal dichalcogenides (TMDCs), of which molybdenum disulfide (MoS$2$) is the most widely studied, are especially attractive [...] Read more.
Two-dimensional (2D) materials with intrinsic atomic-level thicknesses are strong candidates for the development of deeply scaled field-effect transistors (FETs) and novel device architectures. In particular, transition-metal dichalcogenides (TMDCs), of which molybdenum disulfide (MoS$2$) is the most widely studied, are especially attractive because of their non-zero bandgap, mechanical flexibility, and optical transparency. In this contribution, we present an efficient full-wave model of MoS$2$-FETs that is based on (1) defining the constitutive relations of the MoS$2$ active channel, and (2) simulating the 3D geometry. The former is achieved by using atomistic simulations of the material crystal structure, the latter is obtained by using the solver COMSOL Multiphysics. We show examples of FET simulations and compare, when possible, the theoretical results to the experimental from the literature. The comparison highlights a very good agreement. Full article
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Open AccessArticle
Real-Time and Robust Hydraulic System Fault Detection via Edge Computing
Appl. Sci. 2020, 10(17), 5933; https://doi.org/10.3390/app10175933 (registering DOI) - 27 Aug 2020
Abstract
We consider fault detection in a hydraulic system that maintains multivariate time-series sensor data. Such a real-world industrial environment could suffer from noisy data resulting from inaccuracies in hardware sensing or external interference. Thus, we propose a real-time and robust fault detection method [...] Read more.
We consider fault detection in a hydraulic system that maintains multivariate time-series sensor data. Such a real-world industrial environment could suffer from noisy data resulting from inaccuracies in hardware sensing or external interference. Thus, we propose a real-time and robust fault detection method for hydraulic systems that leverages cooperation between cloud and edge servers. The cloud server employs a new approach that includes a genetic algorithm (GA)-based feature selection that identifies feature-to-label correlations and feature-to-feature redundancies. A GA can efficiently process large search spaces, such as solving a combinatorial optimization problem to identify the optimal feature subset. By using fewer important features that require transmission and processing, this approach reduces detection time and improves model performance. We propose a long short-term memory autoencoder for a robust fault detection model that leverages temporal information on time-series sensor data and effectively handles noisy data. This detection model is then deployed at edge servers that provide computing resources near the data source to reduce latency. Our experimental results suggest that this method outperforms prior approaches by demonstrating lower detection times, higher accuracy, and increased robustness to noisy data. While we have a 63% reduction of features, our model obtains a high accuracy of approximately 98% and is robust to noisy data with a signal-to-noise ratio near 0 dB. Our method also performs at an average detection time of only 9.42 ms with a reduced average packet size of 179.98 KB from the maximum of 343.78 KB. Full article
Open AccessArticle
Fault Diagnosis of a Granulator Operating under Time-Varying Conditions Using Canonical Variate Analysis
Energies 2020, 13(17), 4427; https://doi.org/10.3390/en13174427 (registering DOI) - 27 Aug 2020
Abstract
Granulators play a key role in many pharmaceutical processes because they are involved in the production of tablets and capsule dosage forms. Considering the characteristics of the production processes in which a granulator is involved, proper maintenance of the latter is relevant for [...] Read more.
Granulators play a key role in many pharmaceutical processes because they are involved in the production of tablets and capsule dosage forms. Considering the characteristics of the production processes in which a granulator is involved, proper maintenance of the latter is relevant for plant safety. During the operational phase, there is a high risk of explosion, pollution, and contamination. The nature of this process also requires an in-depth examination of the time-dependence of the process variables. This study proposes the application of canonical variate analysis (CVA) to perform fault detection in a granulation process that operates under time-varying conditions. Beyond this, a different approach to the management of process non-linearities is proposed. The novelty of the study is in the application of CVA in this kind of process, because it is possible to state that the actual literature on the theme shows some limitations of CVA in such processes. The aim was to increase the applicability of CVA in variable contexts, with simple management of non-linearities. The results, considering process data from a pharmaceutical granulator, showed that the proposed approach could detect faults and manage non-linearities, exhibiting future scenarios for more performing and automatic monitoring techniques of time-varying processes. Full article
(This article belongs to the Special Issue Incipient Fault Detection and Diagnosis, Fault-Tolerant Control)
Open AccessAbstract
A Sensitive Yellow Fever Virus Entry Reporter Identifies Valosin-Containing Protein (VCP/p97) as an Essential Host Factor for Flavivirus Uncoating
Proceedings 2020, 50(1), 147; https://doi.org/10.3390/proceedings2020050147 (registering DOI) - 27 Aug 2020
Abstract
Flaviviruses are enveloped, arthropod-borne, positive-strand RNA viruses that cause significant human disease. While the basic mechanisms of flavivirus entry and fusion are understood, little is known about the postfusion events that precede RNA replication, such as nucleocapsid disassembly. We recently developed a sensitive, [...] Read more.
Flaviviruses are enveloped, arthropod-borne, positive-strand RNA viruses that cause significant human disease. While the basic mechanisms of flavivirus entry and fusion are understood, little is known about the postfusion events that precede RNA replication, such as nucleocapsid disassembly. We recently developed a sensitive, conditionally replication-defective yellow fever virus (YFV) entry reporter to quantitively monitor the translation of incoming virus particle-delivered genomes. We validated that viral gene expression can be neutralized by YFV-specific antisera and requires known pathways of flavivirus entry; however, as expected, gene expression from the defective reporter virus was insensitive to a small molecule inhibitor of YFV RNA replication. The initial round of viral gene expression was also shown to require: (i) cellular ubiquitylation, consistent with recent findings that dengue virus capsid protein must be ubiquitylated in order for nucleocapsid uncoating to occur, and (ii) valosin-containing protein (VCP)/p97, a cellular ATPase that unfolds and extracts ubiquitylated client proteins from large macromolecular complexes. RNA transfection and washout experiments showed that VCP/p97 functions at a postfusion, pretranslation step in YFV entry. Together, these data support a critical role for VCP/p97 in the disassembly of incoming flavivirus nucleocapsids during a postfusion step in virus entry. Full article
Open AccessArticle
The New Approach to Analysis of Thin Isotropic Symmetrical Plates
Appl. Sci. 2020, 10(17), 5931; https://doi.org/10.3390/app10175931 (registering DOI) - 27 Aug 2020
Abstract
A new approach to solve plate constructions using combined analytical and numerical methods has been developed in this paper. It is based on an exact solution of an equilibrium equation. The proposed mathematical model is implemented as a computer program in which known [...] Read more.
A new approach to solve plate constructions using combined analytical and numerical methods has been developed in this paper. It is based on an exact solution of an equilibrium equation. The proposed mathematical model is implemented as a computer program in which known analytical formulae are rewritten as wrapper functions of two arguments. Partial derivatives are calculated using automatic differentiation. A solution of a system of linear equations is substituted to these functions and evaluated using the Einstein summation convention. The calculated results are presented and compared to other analytical and numerical ones. The boundary conditions are satisfied with high accuracy. The effectiveness of the present method is illustrated by examples of rectangular plates. The model can be extended with the ability to solve plates of any shape. Full article
Open AccessArticle
Ternary Cu(II) Complex with GHK Peptide and Cis-Urocanic Acid as a Potential Physiologically Functional Copper Chelate
Int. J. Mol. Sci. 2020, 21(17), 6190; https://doi.org/10.3390/ijms21176190 (registering DOI) - 27 Aug 2020
Abstract
The tripeptide NH2–Gly–His–Lys–COOH (GHK), cis-urocanic acid (cis-UCA) and Cu(II) ions are physiological constituents of the human body and they co-occur (e.g., in the skin and the plasma). While GHK is known as Cu(II)-binding molecule, we found that urocanic [...] Read more.
The tripeptide NH2–Gly–His–Lys–COOH (GHK), cis-urocanic acid (cis-UCA) and Cu(II) ions are physiological constituents of the human body and they co-occur (e.g., in the skin and the plasma). While GHK is known as Cu(II)-binding molecule, we found that urocanic acid also coordinates Cu(II) ions. Furthermore, both ligands create ternary Cu(II) complex being probably physiologically functional species. Regarding the natural concentrations of the studied molecules in some human tissues, together with the affinities reported here, we conclude that the ternary complex [GHK][Cu(II)][cis-urocanic acid] may be partly responsible for biological effects of GHK and urocanic acid described in the literature. Full article
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Open AccessReview
Membrane-Supported Recovery of Homogeneous Organocatalysts: A Review
Chemistry 2020, 2(3), 742-758; https://doi.org/10.3390/chemistry2030048 (registering DOI) - 27 Aug 2020
Abstract
As catalysis plays a significant role in the development of economical and sustainable chemical processes, increased attention is paid to the recovery and reuse of high-value catalysts. Although homogeneous catalysts are usually more active and selective than the heterogeneous ones, both catalyst recycling [...] Read more.
As catalysis plays a significant role in the development of economical and sustainable chemical processes, increased attention is paid to the recovery and reuse of high-value catalysts. Although homogeneous catalysts are usually more active and selective than the heterogeneous ones, both catalyst recycling and product separation pose a challenge for developing industrially feasible methods. In this respect, membrane-supported recovery of organocatalysts represents a particularly useful tool and a valid option for organocatalytic asymmetric synthesis. However, catalyst leaching/degradation and a subsequent decrease in selectivity/conversion are significant drawbacks. As the effectivity of the membrane separation depends mainly on the size of the catalyst in contrast to the other solutes, molecular weight enlargement of small organocatalysts is usually necessary. In the last few years, several synthetic methodologies have been developed to facilitate their recovery by nanofiltration. With the aim of extending the possibilities for the membrane-supported recovery of organocatalysts further, this contribution presents a review of the existing synthetic approaches for the molecular weight enlargement of organocatalysts. Full article
(This article belongs to the Special Issue Organic Chemistry Research in Hungary)
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Open AccessArticle
CIAA-RepDroid: A Fine-Grained and Probabilistic Reputation Scheme for Android Apps Based on Sentiment Analysis of Reviews
Future Internet 2020, 12(9), 145; https://doi.org/10.3390/fi12090145 (registering DOI) - 27 Aug 2020
Abstract
To keep its business reliable, Google is concerned to ensure the quality of apps on the store. One crucial aspect concerning quality is security. Security is achieved through Google Play protect and anti-malware solutions. However, they are not totally efficient since they rely [...] Read more.
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Open AccessArticle
Experimental Evidence of the Viability of Thermoelectric Generators to Power Volcanic Monitoring Stations
Sensors 2020, 20(17), 4839; https://doi.org/10.3390/s20174839 (registering DOI) - 27 Aug 2020
Abstract
Although there is an important lack of commercial thermoelectric applications mainly due to their low efficiency, there exist some cases in which thermoelectric generators are the best option thanks to their well-known advantages, such as reliability, lack of maintenance and scalability. In this [...] Read more.
Although there is an important lack of commercial thermoelectric applications mainly due to their low efficiency, there exist some cases in which thermoelectric generators are the best option thanks to their well-known advantages, such as reliability, lack of maintenance and scalability. In this sense, the present paper develops a novel application in order to supply power to volcanic monitoring stations, making them completely autonomous. These stations become indispensable in any volcano since they are able to predict eruptions. Nevertheless, they present energy supply difficulties due to the absence of the power grid, the remote access, and the climatology. As a solution, this work has designed a new integral system composed of thermoelectric generators with high efficiency heat exchangers, and its associated electronics, developed thanks to Internet of Things (IoT) technologies. Thus, the heat emitted from volcanic fumaroles is transformed directly into electricity with thermoelectric generators with passive heat exchangers based on phase change, leading to a continuous generation without moving parts that powers different sensors, the information of which is emitted via LoRa. The viability of the solution has been demonstrated both at the laboratory and at a real volcano, Teide (Canary Islands, Spain), where a compact prototype has been installed in an 82 $°$C fumarole. The results obtained during more than five months of operation prove the robustness and durability of the developed generator, which has been in operation without maintenance and under all kinds of meteorological conditions, leading to an average generation of 0.54 W and a continuous emission over more than 14 km. Full article
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Open AccessReview
New-Generation Washing Agents in Remediation of Metal-Polluted Soils and Methods for Washing Effluent Treatment: A Review
Int. J. Environ. Res. Public Health 2020, 17(17), 6220; https://doi.org/10.3390/ijerph17176220 (registering DOI) - 27 Aug 2020
Abstract
Soil quality is seriously reduced due to chemical pollution, including heavy metal (HM) pollution. To meet quality standards, polluted soils must be remediated. Soil washing/soil flushing offers efficient removal of heavy metals and decreases environmental risk in polluted areas. These goals can be [...] Read more.
Soil quality is seriously reduced due to chemical pollution, including heavy metal (HM) pollution. To meet quality standards, polluted soils must be remediated. Soil washing/soil flushing offers efficient removal of heavy metals and decreases environmental risk in polluted areas. These goals can be obtained by using proper washing agents to remove HMs from soil. These washing agents should not pose unacceptable threats to humans and ecosystems, including soil composition. Currently, it is desirable to use more environmentally and economically attractive washing agents instead of synthetic, environmentally problematic chemicals (e.g., ethylenediaminetetraacetic acid (EDTA)). The usefulness of novel washing agents for treatment of heavy metal-contaminated soils is being intensively developed, in terms of the efficiency of HM removal and properties of washed soils. Despite the unquestionable effectiveness of soil washing/flushing, it should be remembered that both methods generate secondary fluid waste (spent washing solution), and the final stage of the process should be treatment of the contaminated spent washing solution. This paper reviews information on soil contamination with heavy metals. This review examines the principles and status of soil washing and soil flushing. The novel contribution of this review is a presentation of the sources and characteristics of novel washing agents and chemical substitutes for EDTA, with their potential for heavy metal removal. Methods for treating spent washing solution are discussed separately. Full article
Open AccessReview
Organoids in Translational Oncology
J. Clin. Med. 2020, 9(9), 2774; https://doi.org/10.3390/jcm9092774 (registering DOI) - 27 Aug 2020
Abstract
Translational medicine aims to translate the most promising preclinical research into clinical practice. Oncology is a continuously growing medical field: the scientific research on cancer biology is currently based on in vitro experiments, carried out on tissue culture plates (TCPs) and other 2D [...] Read more.
Translational medicine aims to translate the most promising preclinical research into clinical practice. Oncology is a continuously growing medical field: the scientific research on cancer biology is currently based on in vitro experiments, carried out on tissue culture plates (TCPs) and other 2D samples. In this context, 3D printing has greatly improved the biofabrication of new biological matrices that mimic the extracellular environments, which may characterize healthy from cancerous tissues. Organoids have recently been described in several reports on scientific literature. The term that better describes such organoids-based tumoral tissues is “tumoroids”. Tumoroids are substantially “tumor-like organoids”, typically deriving from primary tumors harvested from patients. This topical review aims to give an update on organoids applied in translational medicine, paying specific attention to their use in the investigation of the main molecular mechanisms of cancer onset and growth, and on the most impacting strategies for effective targeted therapies. Full article
(This article belongs to the Special Issue Frontiers in Oral Cancer—Basic and Clinical Sciences)
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Open AccessArticle
Entire Magnetic Integration Method of Multi-Transformers and Resonant Inductors for CLTLC Resonant Converter
Electronics 2020, 9(9), 1386; https://doi.org/10.3390/electronics9091386 (registering DOI) - 27 Aug 2020
Abstract
An entire magnetic integration methodology of high efficiency printed circuit board (PCB) winding transformer for CLTLC (capacitor-inductor-transformer-inductor-capacitor) resonant converter is presented. All magnetic components in the converter, including two resonant inductors and two transformers, are integrated into an improved EIE (E-type and I-type [...] Read more.
An entire magnetic integration methodology of high efficiency printed circuit board (PCB) winding transformer for CLTLC (capacitor-inductor-transformer-inductor-capacitor) resonant converter is presented. All magnetic components in the converter, including two resonant inductors and two transformers, are integrated into an improved EIE (E-type and I-type and E-type) core structure. According to the matrix transformer concept and uneven winding distribution, the novel structure can be obtained by introducing an air gap to the center core leg. Thus, the magnetizing inductance and leakage inductance of the transformer can be controlled easily through adjusting the air gap reluctances. In addition, both the detailed mathematical analysis and the reluctance model of the transformer have been studied. Furthermore, a four-layer printed circuit board winding structure is chosen. The related winding arrangement is also discussed in depth. Finally, a 1 kW prototype with the presented structure is implemented to verify the validity of the theoretical analysis. Experimental results demonstrate that the proposed structure guarantees high efficiency within the entire load range. Peak efficiency of 96.62% can be ensured. Full article
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Open AccessArticle
Effects of Lonicera japonica Flower Bud Extract on Citrobacter rodentium-Induced Digestive Tract Infection
Medicines 2020, 7(9), 52; https://doi.org/10.3390/medicines7090052 (registering DOI) - 27 Aug 2020
Abstract
Background: Although antibiotic therapy is currently a gold standard for bacterial infections, it is not used for severe diseases like enterohemorrhagic Escherichia coli, in which the Shiga toxin is overproduced by antibiotic action. The Lonicera japonica flower bud (LJF) is an herbal [...] Read more.
Background: Although antibiotic therapy is currently a gold standard for bacterial infections, it is not used for severe diseases like enterohemorrhagic Escherichia coli, in which the Shiga toxin is overproduced by antibiotic action. The Lonicera japonica flower bud (LJF) is an herbal component used against purulent diseases in traditional Japanese and Chinese medicine. We investigated the effects of LJF extract (LJFE) on Citrobacter rodentium-induced digestive tract infection in a mouse model. Methods:Citrobacter rodentium and LJFE were orally administered to C57BL/6 mice. The survival rate and bacterial colonization in the large intestine, mesenteric lymph node, and blood of mice were evaluated. Cytokines secreted from intraperitoneal macrophages of LJFE-treated mice were measured using ELISA. Moreover, the phagocytic activity of intraperitoneal macrophages against Citrobacter rodentium was compared between LJFE- or chlorogenic acid (CGA)-treated mice. Results: LJFE significantly increased the survival rate and decreased Citrobacter rodentium colonization in mice. Moreover, the values of tumor necrosis factor-α, interleukin-1β, and interferon-γ secreted from macrophages were increased following LJFE treatment. While macrophages of LJFE-treated mice showed a significant phagocytic activity, macrophages of CGA-treated mice only showed a phagocytic tendency. Conclusions: LJF may be useful for treating Citrobacter rodentium-induced digestive tract infection. Full article
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Open AccessReview
A Review of Flexible Wearable Antenna Sensors: Design, Fabrication Methods, and Applications
Materials 2020, 13(17), 3781; https://doi.org/10.3390/ma13173781 (registering DOI) - 27 Aug 2020
Abstract
This review paper summarizes various approaches developed in the literature for antenna sensors with an emphasis on flexible solutions. The survey helps to recognize the limitations and advantages of this technology. Furthermore, it offers an overview of the main points for the development [...] Read more.
This review paper summarizes various approaches developed in the literature for antenna sensors with an emphasis on flexible solutions. The survey helps to recognize the limitations and advantages of this technology. Furthermore, it offers an overview of the main points for the development and design of flexible antenna sensors from the selection of the materials to the framing of the antenna including the different scenario applications. With regard to wearable antenna sensors deployment, a review of the textile materials that have been employed is also presented. Several examples related to human body applications of flexible antenna sensors such as the detection of NaCl and sugar solutions, blood and bodily variables such as temperature, strain, and finger postures are also presented. Future investigation directions and research challenges are proposed. Full article
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Open AccessArticle
Development and Characterization of Membranes with PVA Containing Silver Particles: A Study of the Addition and Stability
Polymers 2020, 12(9), 1937; https://doi.org/10.3390/polym12091937 (registering DOI) - 27 Aug 2020
Abstract
Developing technologies for the reduction of biofouling and enhancement of membrane functionality and durability are challenging but critical for the advancement of water purification processes. Silver (Ag) is often used in the process of purification due to its anti-fouling properties; however, the leaching [...] Read more.
Developing technologies for the reduction of biofouling and enhancement of membrane functionality and durability are challenging but critical for the advancement of water purification processes. Silver (Ag) is often used in the process of purification due to its anti-fouling properties; however, the leaching of this metal from a filtration membrane significantly reduces its effectiveness. Our study was designed to integrate the positive characteristics of poly vinyl alcohol (PVA) with the controlled incorporation of nano-scale silver ions across the membrane. This approach was designed with three goals in mind: (1) to improve antifouling activity; (2) to prevent leaching of the metal; and (3) to extend the durability of the functionalized membrane. The fabrication method we used was a modified version of manual coating in combination with sufficient pressure to ensure impregnation and proper blending of PVA with cellulose acetate. We then used the spin coater to enhance the cross-linking reaction, which improved membrane durability. Our results indicate that PVA acts as a reducing agent of Ag+ to Ag0 using X-ray photoelectron spectroscopy analysis and demonstrate that the metal retention was increased by more than 90% using PVA in combination with ultraviolet-photo-irradiated Ag+ reduced to Ag0. The Ag+ ions have sp hybrid orbitals, which accept lone pairs of electrons from a hydroxyl oxygen atom, and the covalent binding of silver to the hydroxyl groups of PVA enhanced retention. In fact, membranes with reduced Ag displayed a more effective attachment of Ag and a more efficient eradication of E. coli growth. Compared to pristine membranes, bovine serum albumin (BSA) flux increased by 8% after the initial addition of Ag and by 17% following ultraviolet irradiation and reduction of Ag, whereas BSA rejection increased by 10% and 11%, respectively. The implementation of this hybrid method for modifying commercial membranes could lead to significant savings due to increased metal retention and membrane effectiveness. These enhancements would ultimately increase the membrane’s longevity and reduce the cost/benefit ratio. Full article
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Open AccessArticle
Carbonate and Oxalate Crystallization by Interaction of Calcite Marble with bacillus subtilis and bacillus subtilisAspergillus niger Association
Crystals 2020, 10(9), 756; https://doi.org/10.3390/cryst10090756 (registering DOI) - 27 Aug 2020
Abstract
Rock surfaces in natural systems are inhabited by multispecies communities of microorganisms. The biochemical activity of microorganisms and the patterns of microbial crystallization in these communities are mostly unexplored. Patterns of calcium carbonate and calcium oxalate crystallization induced by bacteria Bacillus subtilis and [...] Read more.
Rock surfaces in natural systems are inhabited by multispecies communities of microorganisms. The biochemical activity of microorganisms and the patterns of microbial crystallization in these communities are mostly unexplored. Patterns of calcium carbonate and calcium oxalate crystallization induced by bacteria Bacillus subtilis and by B. subtilis together with Aspergillus niger on marble surface in vitro in liquid medium and in humidity chamber—were studied. Phase identification was supported by XRD, SEM, EDXS; metabolite composition was determined by GC–MS. It was found that the activity of B. subtilisA. niger associations significantly differ from the activity of B. subtilis monocultures in the same trophic conditions. The phase composition and the morphology of the forming crystals are determined by the composition of the metabolites excreted by the microorganisms—particularly by the ratio of the concentrations of extracellular polymeric substances (EPS) and oxalic acid in the medium. The acidification activity of micromycetes may suppress the formation of bacterial EPS and prevent the formation of calcite. The present results can be used in the development of biotechnologies using microbial communities. Full article
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Open AccessArticle
Hormone-Independent Mouse Mammary Adenocarcinomas with Different Metastatic Potential Exhibit Different Metabolic Signatures
Biomolecules 2020, 10(9), 1242; https://doi.org/10.3390/biom10091242 (registering DOI) - 27 Aug 2020
Abstract
The metabolic characteristics of metastatic and non-metastatic breast carcinomas remain poorly studied. In this work, untargeted Nuclear Magnetic Resonance (NMR) metabolomics was used to compare two medroxyprogesterone acetate (MPA)-induced mammary carcinomas lines with different metastatic abilities. Different metabolic signatures distinguished the non-metastatic (59-2-HI) [...] Read more.
The metabolic characteristics of metastatic and non-metastatic breast carcinomas remain poorly studied. In this work, untargeted Nuclear Magnetic Resonance (NMR) metabolomics was used to compare two medroxyprogesterone acetate (MPA)-induced mammary carcinomas lines with different metastatic abilities. Different metabolic signatures distinguished the non-metastatic (59-2-HI) and the metastatic (C7-2-HI) lines, with glucose, amino acid metabolism, nucleotide metabolism and lipid metabolism as the major affected pathways. Non-metastatic tumours appeared to be characterised by: (a) reduced glycolysis and tricarboxylic acid cycle (TCA) activities, possibly resulting in slower NADH biosynthesis and reduced mitochondrial transport chain activity and ATP synthesis; (b) glutamate accumulation possibly related to reduced glutathione activity and reduced mTORC1 activity; and (c) a clear shift to lower phosphoscholine/glycerophosphocholine ratios and sphingomyelin levels. Within each tumour line, metabolic profiles also differed significantly between tumours (i.e., mice). Metastatic tumours exhibited marked inter-tumour changes in polar compounds, some suggesting different glycolytic capacities. Such tumours also showed larger intra-tumour variations in metabolites involved in nucleotide and cholesterol/fatty acid metabolism, in tandem with less changes in TCA and phospholipid metabolism, compared to non-metastatic tumours. This study shows the valuable contribution of untargeted NMR metabolomics to characterise tumour metabolism, thus opening enticing opportunities to find metabolic markers related to metastatic ability in endocrine breast cancer. Full article
(This article belongs to the Special Issue Targeting Tumor Metabolism: From Mechanisms to Therapies)
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